Journal: The Thai Journal of Pharmaceutical Sciences

Article Title: Hepatoprotective and hepatoregenerative therapeutic effects of polyherbal medicine Heparmin TM on Rattus norvegicus Wistar with liver fibrosis

doi: 10.56808/3027-7922.2397

Figure Lengend Snippet: Figure 2: Tissue levels of malondialdehyde (MDA) and superoxide dismutase (SOD) activity and clinical parameters. Polyherbal Heparmin normalized SOD activity, decreased oxidative damage marker MDA, prevented biliary complications and hypolipidemia, and normalized serum albumin. Heparmin did not decrease further urine volume. All data were shown as median and 95% confidence interval. Asterisks indicate values statistically different compared to that of normal group

Article Snippet: CCl4 was from Fluka Chemicals (UK), silymarin (#S0292), LOLA (#O1725), goat anti-mouse IgG-AP antibody (#3562), 5-bromo-2’-deoxyuridine (#B5002), tetramisole HCl ((#L9756), BCIP/nitro blue tetrazolium ((#B1911), and Nuclear Fast Red (#N8002) were purchased from Sigma– Aldrich (St. Louis, MO), mouse monoclonal antibody anti-BrdU (#SC-32323) from Santa Cruz Biotechnology (Santa Cruz, CA), Vectamount (#H5000, Vector Labs.), and Superoxide Dismutase Assay Kit (#7500-100-K) from R&D systems (Minneapolis, MN).

Techniques: Activity Assay, Marker

Journal: BMC Neuroscience

Article Title: Sex-specific cypermethrin-induced hippocampal neurotoxicity is associated with alterations in signaling molecules for antioxidant defense, membrane integrity, apoptosis, and GABAergic integrity

doi: 10.1186/s12868-025-00988-y

Figure Lengend Snippet: GnRH ( A ), Na+/K + ATPase ( B ), PGE-2 ( C ), and COX-2 concentrations in the brains of Male and Female Wistar rats exposed to corn oil (control), low dose (6.25 mg/kg), and high dose (12.5 mg/kg) of cypermethrin, respectively. Asterisk (*) indicates significant ( P < 0.05) weight loss. Bars are mean ± SD

Article Snippet: The following kits were used: GnRH (Sandwich ELISA, Kit #MBS269200, MyBioSource), Na+/K+-ATPase (Colorimetric, Kit #E-BC-K022-M, Elabscience), PGE2 (EIA Kit #ADI-900-001, Enzo Life Sciences), COX-2 (ELISA, Kit #MBS7254655, MyBioSource).

Techniques: Control

Journal: Frontiers in Immunology

Article Title: Synergistic effects of platelet-rich fibrin and CTLA4Ig gene-transfected porcine skin on accelerating wound healing in a rat model of deep second-degree burns: a mechanistic study

doi: 10.3389/fimmu.2025.1756818

Figure Lengend Snippet: Temporal expression profiles of antioxidant enzymes CAT and SOD1. Immunofluorescence staining of (A) Catalase (CAT, red) and (B) Superoxide Dismutase 1 (SOD1, red) in wound tissues from the Vaseline group, PRF group, Pigskin group, and PRF+pig skin group at days 4, 7, 14, and 21 post-treatment. Cell nuclei are counterstained with DAPI (blue). The PRF+pig skin group shows the most pronounced and sustained enhancement in the expression of both antioxidant enzymes, particularly during the proliferative and remodeling phases (D7–D21), indicating a reinforced antioxidant defense system. Scale bar = 100 μm.

Article Snippet: After deparaffinization and antigen retrieval (similar to IHC), sections were permeabilized with 0.1% Triton X-100, blocked with 5% BSA, and incubated overnight at 4 °C with primary antibodies against CAT (1:200, Proteintech) and SOD1 (1:200, Proteintech).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Impact of adropin on superoxide dismutase expression in pancreatic β‐cells of normoglycemic and diabetic rats. (A) Immunofluorescence labeling with anti‐superoxide dismutase antibody and anti‐insulin antibodies showed expression of superoxide dismutase in pancreatic β‐cells. (B) Quantification of the histological analysis showed a significant (*** p < 0.001) decrease in superoxide dismutase distribution in pancreatic endocrine cells and a significant (**** p < 0.0001) decrease in its localization in β‐cells of rats with diabetes when compared to the normal group. n = 6. Scale bar: 50 μm. Data analysis was done using the ANOVA test.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Expressing, Immunofluorescence, Labeling

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Effect of adropin on superoxide dismutase expression in pancreatic α‐cells of normal and diabetic rats. (A) Immunofluorescence staining using anti‐superoxide dismutase and anti‐glucagon antibodies showed expression of superoxide dismutase in pancreatic α‐cells. (B) Quantification of the histological analysis showed a significant (** p < 0.01) decrease in superoxide dismutase distribution in the pancreatic endocrine cells of diabetic rats compared to the normal group. α‐Cell expression of superoxide dismutase did not change with adropin treatment among all groups. n = 6. Scale bar: 50 μm. Data analysis was done using the ANOVA test.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Effect of adropin on catalase, superoxide dismutase and total glutathione activities in the serum samples of healthy and diabetic rats. (A) Catalase activity was significantly decreased in diabetic rats compared to normal controls. Adropin slightly increased catalase in DMT group. (B) Superoxide dismutase was slightly increased in DMT compared to the diabetic treated with adropin. (C) Total glutathione was significantly raised with adropin treatment in DMT compared to DMUT. n = 4–6. Data analysis was done using the ANOVA test. * p < 0.05.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Activity Assay